Immunogenicity of a DNA Vaccine Encoding Ag85a-Tb10.4 Antigens from Mycobacterium Tuberculosis

Authors

  • Amir Teimourpour Department of Epidemiology and Biostatistics, Tehran University of Medical Science, Tehran, Iran
  • Mohsen Arzanlou Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
  • Roghayeh Teimourpour Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
  • Samira Rashidian Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
  • Zahra Meshkat Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract:

Background: Tuberculosis is a life threatening disease that is partially prevented by BCG vaccine. Development of more effective vaccines is an urgent priority in TB control. Ag85a and Tb10.4 are the members of culture filter protein (CFP) of M. tuberculosis that have high immunogenicity. Objective: To analyze the immunogenicity of Ag85a-Tb10.4 DNA vaccine by enzyme-linked immunosorbent assay (ELISA). Methods: In this study a previously described plasmid DNA vaccine encoding Ag85a-Tb10.4 was used to examine its capability in the stimulation of immune responses in an animal model. Female BALB/c mice were vaccinated with 100 μg of purified recombinant vector intramuscularly 3 times at two-week intervals and the levels of five cytokines including IFN-γ, IL-12, IL-4, IL-10 and TGF-β were measured. Results: The levels of IFN-γ and IL-12 for the mice following immunization with Ag85A-Tb10.4 was significantly greater than that of the BCG and control group (p

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Journal title

volume 13  issue 4

pages  289- 295

publication date 2016-12-01

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